For an understanding of the development of endocrine research, and the approach to understanding physiology that was necessary, helpful, and effective, reference is made extensively to historical methods that are no longer required. Biosynthetic hormone preparations are now available for human therapy, and species-specific hormone standards are also available for numerous animal species. Much of the early research on anterior pituitary hormones was performed with extracts obtained from animal pituitary glands and with animal models for the specific analytical determination of the concentrations of anterior pituitary hormones in serum, in pituitary tissue, and in some cases by measurement of urinary excretion, for hormones which are excreted during pregnancy, for example.
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